ASSESSMENT OF GENETIC DIVERSITY AMONG MUSTARD (Brassica juncea (L.) Czern & Coss) GENOTYPES USING PCR BASED DNA MARKERS
Publication Date : 16/01/2015
Mustard [Brassica juncea (L.), 2n=36] is the world’s third most important source of edible oil. An experiment was conducted to study molecular diversity of mustard Brassica juncea (L.) through ISSR primers. The present investigation was undertaken to explore the diversity among Indian mustard genotypes (varieties) using molecular markers. To determine the discriminatory power of the RAPD primers, polymorphism percentage, PIC were calculated. A total of 109 bands were obtained from the ten ISSR primers, out of which 78 showed polymorphism of 71.56% with an average of 7.8 bands per primer. The PIC ranged from 0.75 to 0.993 and genetic similarity varied from 0.478 to 1.000. The cluster analysis revealed two main clusters with highest 58% similarity percentage. The phylogenetic tree constructed by UPGMA method generated two main clusters. Cluster II contains one solitary genotype (Rohini). While cluster II had rest of the genotypes which grouped together in their respective subcluster. Ten ISSR primers selected in the present study gave 71.56% polymorphism. The highest polymorphism percentage was observed with one primer A17898. Therefore, this ISSR primer can be used further for diversity study in different mustard genotypes.
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