Genetic diversity analysis among garlic (Allium sativum L.) genotypes through PCR based molecular markers
Publication Date : 14/12/2014
Garlic is one of the most important spices and condiments for home consumption. It is chiefly used for flavouring and seasoning vegetable dishes. It is commonly termed as “Lasan” and botanically known as Allium sativum L. Sixteen RAPD primers were used to characterize polymorphismand generated a total of 87 bands/alleles out of which 82 bands were polymorphic with an average of 5.43 bands per primer and documented the 92.85 % polymorphism. Calculated values for PIC ranged from 0.473 (OPD-05) to 0.858 (OPG-11) with an average of 0.731 and the average RAPD primer index value was 4.24 per primer. The genotype identification through molecular markers resulted in developing highly diversified map of 16 garlic genotypes. The data revealed that molecular techniques are more accurate than biochemical markers, and can be used for characterization of garlic genotypes, seed purity and for diversity analysis.
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