Effect of Some Plant Growth Regulators on Regenerative Potential of Nodal Explants of Uraria picta (Jacq.) DC.

Publication Date : 01/03/2016

Author(s) :

Shivani Verma , B. D. Vashistha.

Volume/Issue :
Volume 2
Issue 2
(03 - 2016)

Abstract :

Effect of some plant growth regulators have been studied on the regenerative potential of nodal explants of Uraria picta (Jacq.) DC. under in vitro conditions. Multiple shoots were induced on Murashige and Skoog’s medium supplemented with varying concentrations of auxins (IAA, NAA, 2, 4-D, IBA) and cytokinins (BAP, KN) individually as well as in various combinations. The maximum number of shoots were induced on MS medium fortified with BAP (1.0 mg/l) and IAA (1.0 mg/l) in combination. The maximum average shoot length (3.5cm) was observed on BAP (1.0 mg/l). Root induction on in vitro generated shoots was achieved on half-strength MS medium supplemented with IBA (1.0mg/l). The in vitro raised plantlets were acclimatized successfully to pots containing a mixture of autoclaved soil, sand and compost in 2:1:1 ratio with 70% survival. Abbreviations: BAP, 6-Benzyl aminopurine; KN, Kinetin, IAA, Indole-3-acetic acid; IBA, Indole-3-butyric acid; NAA, Naphthalene acetic acid; 2, 4-D, 2, 4-Dichlorophenoxy acetic acid.

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