Evaluation of the antiinflammatory activity of field grown plant and Callus Extracts of Tinospora cordifolia (Willd.) Hook.F.Thoms under in vitro conditions.

Publication Date : 25/02/2016

Author(s) :

Shanthi Vellaiyappan .

Volume/Issue :
Volume 2
Issue 2
(02 - 2016)

Abstract :

The investigation is aimed to carry out the anti inflammatory activity of ethanol extracts of field grown leaf and leaf derived callus of Tinospora cordifolia (Willd.) Hook.F.Thoms (Menispermaceae).Callus was initiated from the leaf explants of Tinospora cordifolia cultured on MS medium supplemented with auxins and cytokinins alone and in combinations. In the case of leaf derived callus, maximum biomass was recorded on medium containing 2,4-D, NAA and BAP combination. Leaf and leaf derived callus extracts were screened for active principles. Berberine an isoquinoline alkaloid is present in Tinospora cordifolia. Berberine content of callus was improved by imposing abiotic stress on their growth. Salt stress (high conc. of NaCl) showed more response compared to other stresses such as light stress (darkness for 16 h) and temperature stress. Ethanol extracts of leaf derived callus grown under salt stress and field grown leaf were used to analyze their anti inflammatory potential under in vitro conditions. It has been observed that the callus extract of leaves show more berberine content and revealed better anti inflammatory activity as compared to in vivo plant leaf extract.

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