Assessment of autoantibodies to glutamate decarboxylase and anti-insulin in spontaneous diabetic dogs and experimental diabetic rat model

Publication Date : 08/03/2016

Author(s) :

Deepa P M* , Dimri.U , Devi Gopinath , Yatoo.M.I.

Volume/Issue :
Volume 2
Issue 3
(03 - 2016)

Abstract :

The aim of the study was to determine autoantibodies to GAD and insulin in diabetic dogs and diabetes induced wistar rat model. Eight diabetic dogs were used for the study. While,eight clinically healthy dogs were used as controls. Six rats were injected with streptozotocin 40 mg/kg body weight intraperitoneally for 5 consecutive days to induced diabetes. Another six rats without streptozotocin served as healthy controls. Dogs and wistar rats were screened for autoantibodies to GAD and insulin by using radioimmunoassay. All diabetic dogs shown anti-GAD antibody and while auto-antibody to insulin was shown by only 37.5% of diabetic dogs. None of the diabetic rat revealed auto-antibodies to GAD and insulin. The current study clearly indicates that assessment of anti-GAD antibody could be a novel predictive of diabetes in dogs. Concurrently, non-reactivity of antibodies to GAD and insulin in induced diabetic rats indicates incompatibility of this model to diabetes in dogs.

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