Molecular Identification of Streptomyces clavuligerus and ‎Structure Elucidation of Its Antimicrobial Efficiency

Publication Date : 04/04/2016

Author(s) :

Dina O. Fathy ‎ , ‎Abd-El Gawad M. Hashem‎ , Wafaa N. El-Tayeb‎ , ‎Yasser M. Ragab ‎.

Volume/Issue :
Volume 2
Issue 4
(04 - 2016)

Abstract :

The present study was carried out for screening antimicrobial substance produced by ‎soil Actinomycetes that demonstrated inhibitory effects against pathogenic ‎microorganisms. Eleven Streptomyces sp. isolates were tested as antimicrobial ‎producers, the most potent producer was isolate encoded C10S19 and was identified ‎by molecular techniques as Streptomyces clavuligerus .The ethyl acetate extract was ‎assayed by paper disk method and purified using thin layer chromatography (TLC). ‎The structure elucidation of antimicrobial agent was performed using ultraviolet ‎‎(UV), Infra-red (IR) and Mass spectra. Results revealed that the antagonistic activity ‎of clavulanic acid salt and holomycin produced by Streptomyces clavuligerus were ‎active against Gram positive Staphylococcus aureus ATCC 29213, Gram negative ‎Escherichia coli ATCC 25922 and unicellular fungi Candida albicans ATCC 10231.‎

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