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Volume: 5 Issue: 10
||KINETIC ESTIMATION OF D-XYLOSE BY ELECTRON TRANSFER INDICATOR REACTION WITH Mn(III) PYROPHOSPHATE
KINETIC ESTIMATION OF D-XYLOSE BY ELECTRON TRANSFER INDICATOR REACTION WITH Mn(III) PYROPHOSPHATE
The Mn(III) in the form of pyrophosphate has been used in the present electron transfer indicator reaction with D-xylose for their kinetic estimations using following indicator reaction.
CHO(CHOH)3CH2OH +2Mn(III) + H2O → CHO(CHOH)2CH2OH +HCOOH +2Mn(II)+ 2H+
The rate data of present indicator reactions in the form of decreasing absorbance at 514nm (λ max of Mn(III) pyrophosphate complex) with time given for this indicator reaction have been used for their respective estimation in simulated samples adopting (a) rate constant, (b) variation of absorbance at fixed time (c) variation of time at fixed absorbance of (d) one point and (e) two point methods using respective calibration plots. The consistency and reproducibility of these results have clearly showed that this kinetic estimations of can be used as an alternative/additional analytical method to the existing traditional estimation methods in the present day laboratory conditions and also as advance laboratory experiments
Keywords: Mn(III) pyrophosphate, Kinetic estimation, Electron transfer , D-xylose, Indicator reaction