Volume:1 Issue:1 Dec ' 2014
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Paper Title |
Abstract |
Page No |
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| 1 |
Genetic diversity analysis among garlic (Allium sativum L.) genotypes through PCR based molecular markers
Author(s):
D.A.Muthamizh.
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1-6 |
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Genetic diversity analysis among garlic (Allium sativum L.) genotypes through PCR based molecular markers
Abstract
Garlic is one of the most important spices and condiments for home consumption. It is chiefly used for flavouring and seasoning vegetable dishes. It is commonly termed as “Lasan” and botanically known as Allium sativum L. Sixteen RAPD primers were used to characterize polymorphismand generated a total of 87 bands/alleles out of which 82 bands were polymorphic with an average of 5.43 bands per primer and documented the 92.85 % polymorphism. Calculated values for PIC ranged from 0.473 (OPD-05) to 0.858 (OPG-11) with an average of 0.731 and the average RAPD primer index value was 4.24 per primer. The genotype identification through molecular markers resulted in developing highly diversified map of 16 garlic genotypes. The data revealed that molecular techniques are more accurate than biochemical markers, and can be used for characterization of garlic genotypes, seed purity and for diversity analysis.
| 2 |
GENETIC DIVERSITY ANALYSIS OF COTTON (Gossypium hirsutum L.) GENOTYPES USING ISSR MARKERS
Author(s):
Srushti Parkhiya , K. Gohel , Dr. D.R. Mehta.
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7-19 |
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GENETIC DIVERSITY ANALYSIS OF COTTON (Gossypium hirsutum L.) GENOTYPES USING ISSR MARKERS
Abstract
Cotton (Gossypium hirsutum L. 2n=52, family: Malvaceae) is one of the most important commercial fiber and oil yielding crop in India. Genetic variability and relationships among fifteen cotton genotypes were investigated using nine ISSR primers. A total of 86 bands were obtained from nine ISSR primers, out of which 54 showed polymorphism of 62.79 % with an average six bands per primer. The PIC ranged from 0.8616 to 0.9090 and genetic similarity varied from 0.60 to 0.917. The cluster analysis revealed two main clusters with highest 66 % similarity percentage. Cluster I consisted of only one genotype GJHV-460, while cluster II consisted of rest of the genotypes grouped together in their respective subclusters. Large numbers of single type of markers could be screened for genetic diversity in cotton genotypes and utilized diverse genotypes in crop improvement programme to enhance crop productivity of cotton.
| 3 |
DNA profiling and phylogenetic relationship among common bean (Phaseolus Vulgaris L.) Genotypes using RAPD markers
Author(s):
V.Ramchandran.
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20-24 |
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DNA profiling and phylogenetic relationship among common bean (Phaseolus Vulgaris L.) Genotypes using RAPD markers
Abstract
Common bean (Phaseolus vulgaris L.; Fabaceae) is a worldwide interesting crop as both grain legume and fresh vegetable. An understanding of genetic diversity is essential for proper utilization of genotypes in the target oriented research programmes. 15 RAPD primers generated a total of 85 bands/alleles out of which 56 bands were polymorphic with an average of 4 bands per primer and documented the 58.24 % polymorphism. Calculated values for PIC ranged from 0.142 (OPB-09) to 0.874 (OPD-07) with an average of 0.698 and the average RAPD primer index value was per primer. The genotype identification through molecular marker resulted in developing highly diversified dendrogram of 12 common bean genotypes. The data revealed that molecular techniques are more precise and more accurate & can be used for genetic diversity analysis of common bean genotypes.
| 4 |
GENETIC DIVERSITY ANALYSIS AMONG SOYBEAN [Glycine max (L.) Merr.] GENOTYPES THROUGH ISSR MOLECULAR MARKERS
Author(s):
P.C. Agrawal.
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25-30 |
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GENETIC DIVERSITY ANALYSIS AMONG SOYBEAN [Glycine max (L.) Merr.] GENOTYPES THROUGH ISSR MOLECULAR MARKERS
Abstract
Soybean is considered as the most despised member of oilseeds and pulses in our country. The 15 ISSR primers were screened to generate 95 bands in which 74 bands were polymorphic with an average of 4.94 bands per primer. The similarity coefficient of clusters analysis was ranged from 60 to 84% for ISSR. The pooled study of ISSR generated clustering pattern which seem to be similar as ISSR clustering pattern. The ISSR primers (UBC-841, UBC-845, UBC-848 and UBC-857) produced genotype specific bands and discriminated six, four and four genotypes among all 16 soybean genotypes respectively.
| 5 |
ASSESSMENT OF GENETIC DIVERSITY AMONG MUSTARD (Brassica juncea (L.) Czern & Coss) GENOTYPES USING PCR BASED DNA MARKERS
Author(s):
Krishna Gohel , Dr.D.R Mehta.
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31-37 |
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ASSESSMENT OF GENETIC DIVERSITY AMONG MUSTARD (Brassica juncea (L.) Czern & Coss) GENOTYPES USING PCR BASED DNA MARKERS
Abstract
Mustard [Brassica juncea (L.), 2n=36] is the world’s third most important source of edible oil. An experiment was conducted to study molecular diversity of mustard Brassica juncea (L.) through ISSR primers. The present investigation was undertaken to explore the diversity among Indian mustard genotypes (varieties) using molecular markers. To determine the discriminatory power of the RAPD primers, polymorphism percentage, PIC were calculated. A total of 109 bands were obtained from the ten ISSR primers, out of which 78 showed polymorphism of 71.56% with an average of 7.8 bands per primer. The PIC ranged from 0.75 to 0.993 and genetic similarity varied from 0.478 to 1.000. The cluster analysis revealed two main clusters with highest 58% similarity percentage. The phylogenetic tree constructed by UPGMA method generated two main clusters. Cluster II contains one solitary genotype (Rohini). While cluster II had rest of the genotypes which grouped together in their respective subcluster. Ten ISSR primers selected in the present study gave 71.56% polymorphism. The highest polymorphism percentage was observed with one primer A17898. Therefore, this ISSR primer can be used further for diversity study in different mustard genotypes.
