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Paper Title |
Abstract |
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| 1 |
Formation of Persister in Klebsiella pneumoniae: Recombinant Expression and Purification of hipA and hipB proteins from K. pneumoniae
Author(s):
Nagendra Singh , Ritu Bhardwaj , imteyaz Qamar.
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1-7 |
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Formation of Persister in Klebsiella pneumoniae: Recombinant Expression and Purification of hipA and hipB proteins from K. pneumoniae
Abstract
Several bacterial populations areknownto be containing some fraction of cells which survive exposure to antibiotics and harsh environment, are called as persister cells. This fraction of cells is very small generally ranging from 10-7 to 10-5. The mechanism of persister formation is not yet clearly understood although expression of toxin-antitoxin (TA) pairs of proteins has been found to be associated with persister formation. Klebsiella pneumoniae is also shown to produce persister cells by prolonged exposure to ampicillin.In this study, we have identified a pair of proteins, hipA and hipB, of TA system in Klebsiella pneumoniae. The proteins have 70% and 60% sequence similarity respectively with their homologous proteins from E. coli. hipA and hipB associate together to regulate survival of persister cells by binding to DNA in unfavourable conditions. Both hipA and hipB proteins from Klebsiella pneumoniae were cloned, expressed and purified. The clones were over expressed in fusion with His-tag in E. coli strains BL-21 (DE3). The purification was done using Immobilized metal affinity chromatography having Ni-NTA matrix. Sequence analysis by in-silico methods shows that hipA and hipB from K. pneumoniae may have different pattern of oligomer formation and DNA recognition than their counterparts in E. coli.
| 2 |
Time Evolution of chlorophyll content in Ocimum Tenuiflorum
Author(s):
R. Jayakrishnan , Tiju Joseph Mathew.
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8-10 |
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Time Evolution of chlorophyll content in Ocimum Tenuiflorum
Abstract
Ocimum Tenuiflorum locally known as “Tulsi” is a widely used indigenous remedy to several ailments in our households. We have studied the evolution of chlorophyll content in the leafs of this plant over time in a day. We observe that the chlorophyll content reaches it maximum during noon time. This suggests that the leaf extract may be of higher medicinal value when extracted during the noon time. This also provides a scientific explanation to the age old practice in our homes of not using the Tulsi leafs for any purpose after sunset.
| 3 |
Phytochemical composition and Total Antioxidant Activity (TAA) of Functional Food Supplements
Author(s):
Dr. Krishnaja. U , Dr. Mary Ukkuru. P .
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11-18 |
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Phytochemical composition and Total Antioxidant Activity (TAA) of Functional Food Supplements
Abstract
Functional foods that contain significant amounts of bioactive components may provide desirable health benefits beyond basic nutrition and play important roles in the prevention of chronic diseases. Among the modifiable lifestyle-related factors, the main role is played by diet showing an inverse association between the intake of antioxidant-rich food and the risk of diseases. The present study explored the various phytochemical contents of the developed Functional Food Supplements (FFS) and thereby exhibited their combined effect on the total antioxidant activity. Among the different solvent media used to study the total antioxidant activity of the functional food supplements, aqueous ethanol showed the highest for supplement I (1.09 µg / g) while absolute ethanol extracted the maximum for supplement II (0.83 µg/g). The phytochemical content of the two supplements also showed significant variations due to the differences in their processing techniques.
| 4 |
Studies on stability of seedling characteristics in rice
Author(s):
R.D.S. YADAV.
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19-21 |
pdf |
Studies on stability of seedling characteristics in rice
Abstract
Fifteen genetically diverse and promising varieties of rice were studied for their seedling characteristics viz.,coleoptile length, root length, soot length, seedling vigour index, root volume and seed yield under four agro –eco systems in order to identify their suitability under direct seeding. Genotype x environment interaction and it’s both linear and non-linear components were found significant for all seedling characteristics and seed yield under studied. Among the genotypes especially developed for upland condition, the Shushksmrat, Baranideep and Vasumathi showed good yield along with higher positive intercept ‘a’ value indicating their stability over environments though the highest yielder was MTU 7029 followed by Swarna Sub 1. Besides, varieties which showed the highest ’a’ values for component characters of effective root system viz., root volume (NDR 359) should prominently be involved in crossing with good characteristics varieties which expressed consistency over environments. Further, the varieties viz., NDR 97, NDR 359 and NDR 2064 showed high seed yield and positive intercept ‘a’ value could successfully be exploited for direct seeding by using drum seeder in rice.
| 5 |
Fruit Consumption Pattern and its Determinants among College Going Girls in Alappuzha
Author(s):
Bhagya D , Shafna S.
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22-33 |
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Fruit Consumption Pattern and its Determinants among College Going Girls in Alappuzha
Abstract
A joint WHO/FAO expert consultation report on diet, nutrition and prevention of chronic diseases sets population nutrient goals and recommends intake of minimum gram of 400 g of fruits per day. Fresh fruits are rich sources of micronutrients and macronutrients with myriad of health benefits. The study aims to find the fruit consumption pattern and awareness among college going girls. Majority (68.57%)were not having daily consumption of fruits as influenced by family fruit intake, income and level of education. Majority (51.42%) were only having 50-60g of fruit/day which was far below the RDA indicating their poor nutritional status and serious concern. More girls (51.4%) were preferring citrus fruits due to its juiceness. Body fat and fruit intake was negatively correlated. Banana was the most commonly consumed fruit as it is economical. The major determinants influencing fruit consumption among college going girls was found to be convenience (28.57%), taste (25.71%), price (17.14%) colour (8.57%) and smell (5.71).42.85% girls were not having awareness on nutritional benefits of fruit intake which suggests the need for intervention programmes in future.
Key Words: Fruit consumption, college girls, determinants, frequency, awareness
| 6 |
CHITOSAN -WHEY PROTEIN COMPLEX (CS-WP) AS DELIVERY SYSTEMS TO IMPROVE BIOAVAILABILITY OF IRON
Author(s):
Zakaria M. R. Hassan , El-Sayed M. M , Heba H. Salama , Mevat I. Foda , Awad R. A..
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34-46 |
pdf |
CHITOSAN -WHEY PROTEIN COMPLEX (CS-WP) AS DELIVERY SYSTEMS TO IMPROVE BIOAVAILABILITY OF IRON
Abstract
Use of whey proteins as a natural nano-capsular vehicle to carry and improve the bioavailability of iron was investigated in this study. Nanoparticles of whey protein concentrate-chitosan (CS-WPC) complex were prepared with the aim of developing a biocompatible carrier for the oral administration of iron as a nutraceuticals. Effects of pH, concentration of native CS-WPC and iron on the nanoparticles with sodium tripolyphosphate (TPP) prepared by ionic gelation were investigated. CS-WPC were loading with different iron concentration namely; ferrous sulphate. The surface charge of the particles was positive and negative that strongly pH dependent and showed positive charge after iron loading at low protein concentration and was negative at 8 and 12 % when the pH increased to 5.5. The association efficiency (AE) and loading efficiency (LE) of CS-WPC nanoparticles was highly sensitive to formulation pH. This adsorption can be mainly attributed to electrostatic, hydrophobic interactions and hydrogen bonding between WPC and CS. The iron release experiments showed that the nanoparticles prepared with native WPC had favorable properties to resist acid and pepsin degradation in simulated gastric conditions. When transferred to simulated intestinal conditions, the WPC shells of the nanoparticles were not degraded by pancreatin showing the same results with and without enzymes after 6 h. CS-WPC iron nanoparticles at level 0, 3, 6, 9, 12 mg/g protein showed very high bioavailability after evaluated in simulated gastric and intestinal fluids in the presence or absence of the enzymes.
Keywords: Nanoparticles; Whey proteins concentrate; Chitosan; Iron bioavailability
| 7 |
Evaluation of Inter Species Relationship of Cinnamomum Species from Tirunelveli Hills Using RAPD
Author(s):
N.Nirmal kumar.
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47-50 |
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Evaluation of Inter Species Relationship of Cinnamomum Species from Tirunelveli Hills Using RAPD
Abstract
Abstract
Genetic inter-relationship of nine Cinnamomum species was estimated using randomly amplified polymorphic DNA (RAPD) markers. The 17 selected RAPD primers out of 5 primers were amplified in all cinnamon species. Cinnamomum verum has very close similarity (77%) with C. citronella and another two species of C. camphora and C. glucens. Our results suggested that genetic relationships in Cinnamomum species using RAPD banding data may be useful for plant improvement and an efficient way to conserve genetic resources of Cinnamomum species, in addition to their effective medicinal uses.
Key Words: Lauraceae; Cinnamomum; DNA; RAPD; Southern India
| 8 |
INFLUENCE OF INTERCROPPINGDHAINCHA (Sesbaniaaculeata) ONAGRONOMIC YIELD OF DIRECT SEEDED RICE
Author(s):
Kalaiyarasan V , SubbalakshmiLokanadhan.
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51-54 |
pdf |
INFLUENCE OF INTERCROPPINGDHAINCHA (Sesbaniaaculeata) ONAGRONOMIC YIELD OF DIRECT SEEDED RICE
Abstract
Intercropping of green manure in wet seeded rice for a brief spell of 35 to 40days and incorporating in rice crop would encash the least detrimental effect of early shading, besides ensuring addition of green manure to rice crop. The field experiments were conducted in the wetland farms of Tamil Nadu Agricultural University, Coimbatore to evaluate the effect of Dhaincha incorporation on the agronomic yield of rice. The experiment was laid out in randomized block design with ten treatments. Accumulation of biomass in Dhaincha was distinctly increased when Dhainchawas sown in rice withDrum seeder direct seeding and two rows of Dhaincha in between rice (1:2) recorded higher biomass of 18.9 t ha-1 followed by rice direct seeding with drum seeder + one row Dhaincha in between (1:1) recording 14.2 t ha-1. Rice with green manure intercropping and incorporating in rice Direct seeding with drum seeder + two rows of Dhaincha in between (1:2) recordedhigher grain (7630 kg ha-1) and straw (8900 kg ha-1) yield respectively. The dynamics of Dhaincha intercropping and incorporation in direct seeded rice crop, its influence on agronomic yield, has been discussed in this paper.
| 9 |
A simple approach to study the performance of electron Monte Carlo algorithm in cancer treatment using medical Linear Accelerator
Author(s):
Niyas P , Abdullah K.K , Noufal M.P.
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55-64 |
pdf |
A simple approach to study the performance of electron Monte Carlo algorithm in cancer treatment using medical Linear Accelerator
Abstract
Radiation therapy is a cancer treatment technique using ionizing radiations. This is possible by a medical Linear Accelerator (LINAC) along with a Treatment Planning System (TPS). Most commercial TPS incorporates complex treatment planning algorithms. The electron Monte Carlo (eMC) dose calculation algorithm of the EclipseTM TPS is based upon Monte Carlo simulation of the LINAC head and modelling of the beam characteristics. In this study, a simple and direct dosimetric method is introduced to check the performance of this algorithm for clinical use. The eMC calculated values of different dosimetric quantities such as treatment monitor units (MU), output factors (O.F) and cutout factors are compared with the measured data for available applicators, energy and selected Source to Surface Distances (SSD). Measurements were performed in Varian clinac - iX LINAC using plastic water phantom and 0.13 cm3 ionization chamber. The cutouts, made up of an alloy cerrobend, were prepared in various sizes and shapes. Our results show that the eMC calculated relative O.F for all applicators at different SSDs agree with the measurement within 3%. A clinically acceptable variation of 3% is observed in the cutout factors for square cutouts with sizes of 4 x 4 cm2 or larger. The comparison of irregular clinical cutouts in low and high energy beams with nominal and extended SSDs, yields that Monte Carlo calculated MU matches with corrected MU within 4%. Our results indicate that eMC algorithm shows acceptable agreement with the measured data. The implemented new method of direct measurement successfully validates the eMC for clinical use.
Keywords: Linear accelerator; electron Monte carlo (eMC); monitor units; electron cutout; out put factor.
| 10 |
Nitrogen uptake, quality parameters and post harvest soil status of transplanted finger millet by organic, inorganic and biofertilizers
Author(s):
S Poorna Teja , K V RAMANA MURTHY.
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65-70 |
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Nitrogen uptake, quality parameters and post harvest soil status of transplanted finger millet by organic, inorganic and biofertilizers
Abstract
A field experiment conducted during rabi, 2013-14 on sandy loam soils of Agricultural college farm, Bapatla in transplanted white finger millet to observe the nitrogen use efficiency by organic, inorganic and biofertilizers. The experiment was laid out in Randomized Block Design with nine treatments replicated thrice. The results showed that the highest grain N-uptake were observed 75 % RDN + 25% N through Vermicompost (T4) and highest straw uptake was recorded with 125% RDN + ST with Azospirillum @ 5kg ha-1 + 0.2% Zn spray at flowering stage. The overall N-uptake was high with 125% RDN + ST with Azospirillum @ 5kg ha-1 + 0.2% Zn spray at flowering stage
| 11 |
STUDIES ON THE SYMPTOMS OF THE NECROTIC WILT OF SUNFLOWER CAUSED BY TOBACCO STREAK VIRUS
Author(s):
Deepa Rani C. V , Dr. Susha S. Thara , Dr. Heera. G , Narmadhavathy S.
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71-75 |
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STUDIES ON THE SYMPTOMS OF THE NECROTIC WILT OF SUNFLOWER CAUSED BY TOBACCO STREAK VIRUS
Abstract
The sunflower necrosis disease of sunflower is one of the important diseases causing heavy losses in edible oil seed in the country. It is an emerging threat for sunflower production system in southern India, reported for the first time at Bagepally region of Kolar district and around Bangalore in Karnataka during 1997. Later, its occurrence was reported from almost all sunflower growing states of India, posing threat to sunflower cultivation. A new virus, imparting necrosis leading to mortality of the plants have been reported. Considering the importance of yellowing and necrosis disease in sunflower, the present study has much relevance and hence will be a stepping stone to etiological aspects of virology. The virus isolates causing necrosis and yellowing respectively have been identified as two different strains of Tobacco Streak Virus (TSV) viz. Isolate-N (I-N) and Isolate-Y (I-Y). The two isolates caused different symptoms at different growth stages of sunflower plants in Kharif and Rabi seasons.
| 12 |
SOIL PROPERTIES AND NUTRIENT UPTAKE INFLUENCED BY ORGANIC MANURES AND MICROBIAL INOCULANTS IN KASTHURI TURMERIC (CURCUMA AROMATICA SALISB.).
Author(s):
Bhende S. S. , P.C. Jessykutty, , G.R. Sulekha , B. K. Jayachandran , Hari Kumar V.
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76-81 |
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SOIL PROPERTIES AND NUTRIENT UPTAKE INFLUENCED BY ORGANIC MANURES AND MICROBIAL INOCULANTS IN KASTHURI TURMERIC (CURCUMA AROMATICA SALISB.).
Abstract
The experiment was conducted at the farm attached to the College of Agriculture, Vellayani (Thiruvananthapuram) during 2010-2011 with the main objective of studying the combined effects of organic manures and microbial inoculants on soil properties and nutrient uptake in kasthuri turmeric (Curcuma aromatica Salisb.). The treatments were based on prestandardised doses and included full dose of individual organic manures FYM (40 t ha-1), Vermicomost (25 t ha-1) and Neemcake (6 t ha-1) in combination a common with prestandardised dose of microbial inoculants {Azospirillum + AMF + Trichoderma + Pseudomonas (mi)}, half dose of above organic manures individually in combination with mi, half FYM dose + quarter dose of Vermicompost and Neemcake in combination with mi and half FYM dose + 1/8 th dose of Vermicompost and Neemcake in combination with mi. The above treatments along with control formed the nine treatments which were repeated thrice in RBD. The post experiment study revealed an improvement in the soil physical and chemical properties in all treatments with a general reduction in soil bulk density and an increase in the water holding capacity of the soil, but no appreciable change in soil pH. An increase in the electrical conductivity and in organic carbon was noticed in all treatments including the control. General increase in available N, P and K in soil were noticed in all treatments. The highest values for nitrogen was recorded in higher dose of Neemcake (6 t ha-1) in combination with mi whereas, available Phosphorus and Potassium were recorded in Vermicomost (25 t ha-1) in combination with mi. Plant analysis revealed that Nitrogen uptake was maximum observed in treatment with full dose application of vermicompost with mi (102.48 t ha-1), whereas maximum Phosphorus uptake was noticed with half FYM 40 t ha-1 + quarter dose of Vermicompost and Neemcake in combination with mi (59.76 t ha-1). Moreover, uptake of Potassium as observed in the treatment in full dose treatments of vermicompot and neemcake (260.88 t ha-1 and 262.56 t ha-1 respectively).
