Volume:5 Issue:2 Mar ' 2019
| Sr No. |
Paper Title |
Abstract |
Page No |
Download |
| 1 |
The effect of intensive growth of Simmental heifers on the abbreviated age of the first insemination.
|
View
|
1-4 |
pdf |
The effect of intensive growth of Simmental heifers on the abbreviated age of the first insemination.
Abstract
ANNOTATION
The article presents the results of the conducted research experiments on the maintenance and feeding of Simmental heifers, from heifers imported from Europe, aged 0-14 months.
SUMMARY
Results of the made scientific research experiments after contents and feedings of calfs of Simmental breed, from heifers brought in from Europe, at the age of 0-14 months are presented in article.
| 2 |
Production of a polypeptide Antimicrobial Compound by Lactic Acid Bacteria Isolated from Rhizospheric Soil of Egyptian Organic Farms
|
View
|
5-13 |
pdf |
Production of a polypeptide Antimicrobial Compound by Lactic Acid Bacteria Isolated from Rhizospheric Soil of Egyptian Organic Farms
Abstract
Abstract
The current study aimed to isolation of lactic acid bacteria (LAB) showing antimicrobial activity mainly against some fungal and bacterial pathogens. A total of 41 LAB strains were isolated from the rhizosphere of healthy and diseased tomato and phaseolus plants using MRS agar medium supplemented with bromocresol green as an indicator of lactic acid production. The isolated bacterial strains were screened for antimicrobial activity against Staphylococcus aureus ATCC 2913 (G +ve), Pseudomonas aeruginosa ATCC 27953 (G -ve) and Mucor racemosus (phytopathogenic fungus). Among the tested LAB isolates, 32 of them showed antimicrobial activity against the tested pathogens with variable potency. However, LAB isolate No.38 exhibited the highest antimicrobial activity against the indicator strains and was the only strain showing antifungal activity against Mucor racemosus. According to 16S rDNA sequence and phylogenetic analysis, strain No.38 showed the highest similarity (98%) with Enterococcus faecium and was designed as Enterococcus faecium MN 38 (accession No.MK280754). Investigation of the effect of various physiological parameters revealed production of the bioactive agents by E. faecium MN 38 at temperature range 30-37 °C, pH range 4-7. In addition, strain MN 38 was able to tolerate NaCl concentration up to 4%. Finally, the precipitation of the bioactive compound from the culture filtrate by 60-80% ammonium sulphate, suggested the proteinaceous nature of the bioactive agent produced by E. faecium MN 38. The results indicated that plant rhizosphere is a promising source of antimicrobial agents producing LAB with potential application in biocontrol of phytopathogens and soil-born pathogenic bacteria and fungi.
| 3 |
ARTIFICIAL INSEMINATION OF EMU SEMEN DILUTED WITH IMV AND MODIFIED BELTSVILLE POULTRY SEMEN EXTENDERS (MBPSE)
|
View
|
14-20 |
pdf |
ARTIFICIAL INSEMINATION OF EMU SEMEN DILUTED WITH IMV AND MODIFIED BELTSVILLE POULTRY SEMEN EXTENDERS (MBPSE)
Abstract
A study was conducted for insemination of emu semen diluted with IMV poultry semen extender (IMV) and Modified Beltsville Poultry Semen Extender (MBPSE). Ten adult male and fifteen female emu birds aged 3 to 4 years were selected and housed individually in emu unit, University Research Farm, TANUVAS, Chennai – 600051. The male birds were selected based on their readiness in accepting human beings without fear. All the birds were housed properly under standard managemental condition. An Isocaloric and Isonitrogenous standard emu breeder ration was fed to birds and portable drinking water were made available ad libitum. The selected male emus were trained for semen collection by non-teaser method. After collection, the raw semen was diluted with IMV and MBPSE semen extenders at 1:3, 1:4 & 1:5 ratio and stored at 4oC and evaluated all the seminal attribute in 1, 3 and 6 hours interval. The semen diluted 1:3 & 1:4 ratio stored for 1 hour showed better seminal characteristics and chosen for insemination. The female birds were restrained by casting on the floor; the artificial insemination was carried out with diluted semen. The female started laying eggs three to four day after insemination. All the eggs were set for incubation. A significant (P<0.01) difference was observed in fertility of female emu birds inseminated with extended semen and the IMV showed better fertility and hatchability than MBPSE and the embryonic mortality did not showed any difference. It is concluded that the emu semen can be stored for a short period of 1 to 3 hour at 1:3 & 1:4 ratio of LSE in 4oC with better seminal attributes for further processing like artificial insemination and cryopreservation.
