Volume:8 Issue:1 Jan ' 2022
||A STUDY ON MOLECULAR IDENTIFICATION OF ORCHID MYCORRHIZAL FUNGI OF NERVILIA CROCIFORMIS
A STUDY ON MOLECULAR IDENTIFICATION OF ORCHID MYCORRHIZAL FUNGI OF NERVILIA CROCIFORMIS
Present study aimed on identification of orchid mycorrhizal fungi (Nervilia crociformis)
using molecular techniques. The terrestrial orchid, Nervilia crociformis, was collected
from natural habitats from various locations of Kemmangundi and Sagar, Karnataka State
and were investigated for mycorrhizal association. Freshly collected root/tuber of Nervilia
crociformis was used for isolation and identification. Pure cultures were obtained by
hyphal tip method and were maintained in PDA slants. Pure cultures were used for
isolation of genomic DNA and the isolated DNA was run in agarose gel electrophoresis.
Polymerized chain reaction (PCR) was performed. The purified PCR product sequences
were determined by the chain termination method using an ABI 3130 Genetic Analyzer.
The ITS sequence data of the isolate from the tuber of Nervilia crociformis showed close
resemblance to Colletotrichum fruticola (Gene Bank Accession No.: KC920479). There
was 98% homology to the above fungus along with Glomerella cingulate (perfect stage of
the above fungus). Colletotrichum fruticola was found in Nervilia crociformis and they
have formed pelotons in cortical cells of the tuber. Thus, the molecular identification study
confirms that fungi Colletotrichum fruticola was isolated from tuber of Nervilia
||Phenotypic characteristics of local poultry (hen and guinea fowl) in Niger
Phenotypic characteristics of local poultry (hen and guinea fowl) in Niger
The present study was initiated to characterise local chicken and guinea fowl populations of Niger in order to provide necessary information to better valorise their breeding. For this porpose, a survey is carried out on a sample of 200 poultry farmers of all species and the characterisation work concerned 400 adult poultry. Thus, a total of 20 villages, spread over 18 communes in the Regis-ER project area, were covered. Several morpho-biometric and qualitative characteristics were assessed in both the local hen and guinea fowl, including the type of plumage, crest, polydactyly, feather distribution, colour of the plumage, tarsus, legs, barbels, skin, earlobe, beak, eggshell and eyes. It emerged from the analysis of results that, majority of the respondents were aged between 30 and 50 years (64%). Their main activity was agriculture (73%). Poultry farming is practiced majority by married women. A total of seven local varieties of chiken were encountered, including Dourgou, Goggori, Gouzou-gouzou, Kollonto, Métis, Populaire and Tchiagara. With the exception of wing length and barb length, the Kolonto variety has the highest average values. The Dourgou variety has much smaller mean values for tarsal length, wing length and wingspan. Five local guinea fowl varieties were observed, namely Angoulou, Hwaraa, Jaa, Koural fataké and Zabako. The Angoulou variety had the highest average values. The lowest live weight values were obtained in Hwaraa (1027.85 g) and Jaa (1057.50 g). In addition, molecular analysis will allow a better assessment of the genome diversity of the local hen and guinea fowl population.
Keywords: Niger, local chicken and guinea fowl, Phenotypic, characteristics]