DNA profiling and phylogenetic relationship among common bean (Phaseolus Vulgaris L.) Genotypes using RAPD markers

Publication Date : 14/12/2014


Author(s) :

V.Ramchandran.


Volume/Issue :
Volume 1
,
Issue 1
(12 - 2014)



Abstract :

Common bean (Phaseolus vulgaris L.; Fabaceae) is a worldwide interesting crop as both grain legume and fresh vegetable. An understanding of genetic diversity is essential for proper utilization of genotypes in the target oriented research programmes. 15 RAPD primers generated a total of 85 bands/alleles out of which 56 bands were polymorphic with an average of 4 bands per primer and documented the 58.24 % polymorphism. Calculated values for PIC ranged from 0.142 (OPB-09) to 0.874 (OPD-07) with an average of 0.698 and the average RAPD primer index value was per primer. The genotype identification through molecular marker resulted in developing highly diversified dendrogram of 12 common bean genotypes. The data revealed that molecular techniques are more precise and more accurate & can be used for genetic diversity analysis of common bean genotypes.


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