Invitro ovicidal activity of Allophyllus cobbe leaf extracts against Haemonchus contortus
Publication Date : 31/03/2015
The anthelmintic activity of methanolic, aqueous and hydroalcoholic extracts of Allophyllus cobbe was assessed invitro using egg hatch assay. Fresh ova of Haemonchus contortus from infected goat were concentrated using faecal flotation method and were washed with normal saline to remove the debris. 100µL of the washing containing approximately 100 ova were transferred to a 6 well plate and equal volume of extracts were added at dilutions of 50, 25, 12.5 and 6.25 mg/ml. Albendazole and ivermectin at dose rates of 1 and 10 mg/ml served as positive control where as normal saline acted as negative control. They were incubated at 28ºC for 48 hours and the number of dead, live or hatched larvae and ova were counted under the microscope to assess the percentage of hatching. The phytochemical analysis of the extracts were done using standard protocols as well as the acute oral toxicity was assessed at the dose rate of 2000mg/kg in rats. The alcoholic extract at dose rates of 50, 25 and 12.5 mg/ml completely inhibited the hatching of ova where as the extract in the dose of 6.25 mg/ml produced only 50% inhibition. The ova were found disintegrated in the treatment groups with 50 and 25 mg/ml and also with the control drugs. None of the extracts showed any toxic reactions during the entire period of observations. From the study it could be concluded that the methanolic extract of Allophyllus cobbe showed potent anthelmintic property and the further isolation and characterization of the extract could prove a lead for the synthesis of a novel anthelmintic molecule.
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