Intraspecific polymorphism in wild and cultured diploid species of Matricaria L Detected by molecular cytogenetic markers, analysis of meiosis and Essential oil composition
Publication Date : 30/04/2015
The comparative studies of diploid Matricaria chamomilla L (2n=18) (Azulena and Sybirskaya bisabololnaya varieties), Matricaria discoidea L. (2n=18) and Matricaria inodora L. (2n=18) were carried out by DAPI/C-banding, FISH with 26S and 5S rDNA probes, the analysis of meiosis and composition of essential oil. Based on DAPI/C banding and FISH with 26S and 5S rDNA probes, all the chromosomes in karyotypes of the studied diploid chamomile species were identified, and generalized idiograms of chromosomes with account of all possibilities of DAPI/C-banding patterns as well as chromosomal localization of 26S and 5S rDNA sites were constructed. The results of comparative analysis of DAPI/C-banding and localization of ribosomal genes in the studied related chamomile species conformed to the hypothesis on monophyletic origin of their genomes. In most cases, we observed the classical type of meiosis with formation of 9 baculiform bivalents. Insignificant percentage of abnormalities suggested genetic stability of genomes of the studied species. The analysis of essential oil composition confirmed that M. discoidea could be used for medical purposes in common with M. chamomillа. The comparative analysis of interspecific karyotype differences and also the qualitative composition of essential oil allowed us to conclude that Md genome took an intermediate position between Mch and Mi genomes, and M. discoidea was considerably closer to M. chamomillа than M. inodora. Keywords C/DAPI-banding, fluorescence in situ hybridization, genome, Matricaria, meiosis.
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