Effect of caprine ovarian follicular fluid on maturation of caprine oocytes in vitro

Publication Date : 27/05/2015


Author(s) :

Dr. Lakshmikanth,T.R , Dr. Devaraj.M. , Krishnaswamy, A.K , Chandrashekara Murthy,V.4.


Volume/Issue :
Volume 1
,
Issue 5
(05 - 2015)



Abstract :

The study was conducted to evaluate the effect of caprine follicular fluid on the maturation of caprine oocytes in vitro. The follicular fluid was collected by aspiration of visible follicles of size >3mm in diameter from slaughter house ovaries. The caprine ovaries were collected immediately after slaughter and cumulus oocyte complexes were retrieved by aspiration followed by slicing , graded and subjected to in vitro maturation in 25, 50 and 100 per cent concentration of caprine follicular fluid. The basic culture medium (Control) comprised of Tissue culture medium (TCM199), BSA (3mg/ml) and Gentamicin (50µg/ml). Only grade A and B were placed in the culture medium in Petri dishes with overlaying mineral oil and incubated for 24 h at 38ºC, 5%CO2 in 95 per cent relative humidity. The maturation was evaluated by cumulus expansion score and extrusion of first polar body. The maturation rate of caprine oocytes in 25,50, and 100 per cent concentration of caprine follicular fluid were 78.50±2.4,77.63± 2.57 and 83.65±3.59 respectively which was significantly higher than those that obtained in control (55.08±3.76). It can be concluded that addition of follicular fluid to culture medium enhances the maturation of caprine oocytes in vitro. Keywords: Follicular fluid, caprine, in vitro, maturation, polar body


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