Isolation and Screening of Indigenous Entomopathogenic Fungi against Sucking Pests of Vegetables

Publication Date : 31/05/2015


Author(s) :

Reji Rani O.P , Shifa B.S. , Soni K. B. , Sudharma K..


Volume/Issue :
Volume 1
,
Issue 5
(05 - 2015)



Abstract :

Geographical variation and strain specificity can alter the host specificity of entomopathogenic fungi. Since entomopathogenic fungi is one of the safe and alternative tool for pest management and management of sucking pests in vegetables often demands repeated use of systemic insecticides, attempts were made to isolate indigenous strains of entomopathogenic fungi specific to sucking pests infesting vegetables grown in Kerala. Infected insects collected from various fields and soils collected from cropped, non cropped forests as well as sacred groves were used for isolation using insect bait method, soil dilution plating and Warcup method. The morphological characters were studied using Motic images and molecular characterization was done using tools like Polymerase Chain Reaction, ITS DNA amplification and sequencing. Among different isolates we could locate three entomopathogenic fungi belonging to Ascomycota coming under the genus Lecanicillium and its two closely related genera Eupenicillium and Simplicillium. Though all the three were infective to sucking pest, the isolate Ls Vs.1, identified as Lecanicillium saksenae was found to be the most promising one. Conidial suspension of L. saksenae at 6.98 X 107 spores ml-1 was found to cause 100 % mortality to Aphis craccivora within 24 hours. In this context we propose Lecanicillium saksenae (ITCC No: of Ls.Vs.1 -7714) collected from Vellayani soil as a potent entomopathogen effective to sucking pests of vegetables. Keywords – entomopathogenic fungi; Lecanicillium saksenae; indigenous isolates; isolation; molecular characterisation


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