Detection of Staphylococcus aureus from clinical and sub clinical bovine mastitis milk samples using chicken egg yolk antibodies (IgY)

Publication Date : 06/07/2015


Author(s) :

Mrs. M. Jagadeeswari , Mr. R. Dhanabalan , Mrs. G. Saranya.


Volume/Issue :
Volume 1
,
Issue 7
(07 - 2015)



Abstract :

The egg yolk IgY antibodies were generated against Staphylococcus aureus strains isolated from clinical bovine mastitis milk samples by immunization in 21 week old white leg horn chickens. The protein content of purified IgY showed a gradual increase in protein concentration from twenty first day of immunization, ranging from 0.1 to 3.7 mg/mL of egg yolk and a single protein band with molecular weight of 180 K.Da was obtained by polyacrylamide gel electrophoresis. The titre of antibody was found to be 1: 10000 on 150th day of immunization estimated by ELISA. The S.aureus IgY was specific to S. aureus antigens and showed no cross reactivity with the whole cell antigens of Streptococcus epidermidis, Escherichia coli and Pseudomonas aeruginosa. The egg yolk generated antibodies were tested for its agglutination with four clinical and fifty six normal milk samples, where three clinical and three normal milk samples proved positive agglutination. Simultaneously culturing method of S.aureus was also performed all through the test for the conformation in the analysis. Key words: Agglutination, Bovine mastitis, S. aureus, Chicken IgY antibodies


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