Molecular Markers: Application in plant improvement programmes

Publication Date : 29/07/2015


Author(s) :

Praneet Chauhan , Pankaj Kumar.


Volume/Issue :
Volume 1
,
Issue 7
(07 - 2015)



Abstract :

The development and use of molecular markers for the detection and exploitation of DNA polymorphism is one of the most significant developments in the field of molecular genetics. The presence of various types of molecular markers, and differences in their principles, methodologies, and applications require careful consideration in choosing one or more of such methods. The idea of using genetic markers appeared very early in literature but the development of electrophoretic assays of isozymes and molecular markers have greatly improved our understanding in biological sciences. Molecular detection of plant pathogens and characterization of genetic variability by using different DNA markers have offered additional tools in the hands of plant pathologists and plant breeders. This article provides detail review for different molecular marker methods: restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), inter-simple sequence repeats (ISSRs), sequence characterized regions (SCARs), sequence tag sites (STSs), cleaved amplified polymorphic sequences (CAPS), microsatellites or simple sequence repeats (SSRs), expressed sequence tags (ESTs) and single nucleotide polymorphisms (SNPs)


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