Solubilisation of Inorganic Phosphates by Microorganisms Isolated from Cold desert Habitat of Populus alba (White Poplar) in Trans Himalayas of Himachal Pradesh of India
Publication Date : 31/07/2015
Phosphate solubilising microorganisms (PSM) were isolated from the natural habitats of Populus alba from Lahaul and Spiti valleys of Himachal Pradesh by pour plating. The PSM formed yellow halos around their colonies on Modified Pikovskaya agar using bromo phenol blue, supplemented with tri calcium phosphate (TCP) as source of insoluble inorganic phosphate. Population enumeration showed the highest count of Phosphate solubilising bacteria (PSB) and fungi (PSF) in the rhizosphere than that of non-rhizosphere. The PSB isolated belonged to genera Bacillus and Micrococcus while the PSF isolated frequently belonged to species of Aspergillus and Penicillium. The isolates with high efficiency for tricalcium phosphate solubilisation, were further tested for their capacities to solubilise Mussoorie Rock Phosphae (MRP), Udaipur Rock Phosphate (URP) and North Carolina Rock Phosphate (NCRP). The bacterial isolate PBC2 (Bacillus) was reported to be highly efficient while amongst fungi PFC6 (Aspergillus niger) was solubilising maximum insoluble phosphate.
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