FRACTIONATION AND PURIFICATION OF BIOACTIVE PEPTIDES IN EXCRETORY/SECRETORY PRODUCTS OF THIRD INATAR LARVAE OF CHRYSOMYA MEGACEPHALA (CALLIPHORIDAE : DIPTERA)
Publication Date : 31/08/2015
The beneficial effects of maggots on wounds have been attributed to various mechanisms notably the debridement (degradation) of necrotic tissue.It was demonstrated that the maggots ES exhibit antibacterial actions against both Gram-positive and Gram-negative bacteria, including MRSA, Escherichia coli and Pseudomonas aeruginosa.The present study revealed the presence of 40 protein fractions in excretory/secretory products of third instar larvae of Chrysomya megacephala, only 3 protein fractions showed antibacterial activity against Bacillis subtilis, number 22,23 and 24. The separated protein fraction number 22 showed protein bands at 28 and 31 KDa protein, separated protein fraction number 23 showed protein bands at 17, 24 and 28 KDa and separated protein fraction number 24 showed protein band at 26 KDa.
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