Genetic diversity analysis of blackgram [Vigna mungo (L.) Hepper] using morphological and molecular markers

Publication Date : 28/08/2015


Author(s) :

S. K. Noren , Artibashisha Hijam Pyngrope , Tyagi Wricha , Devyani Sen , V. K. Khanna , A. Pattanayak.


Volume/Issue :
Volume 1
,
Issue 8
(08 - 2015)



Abstract :

A total of 30 blackgram [Vigna mungo (L.) Hepper] genotypes including five locally adapted varieties, twenty three advanced breeding lines and two local germplasm were evaluated for genetic diversity and relatedness with 21 morphological markers and 12 SSR markers. Genotypes viz., Uttara-IVT, Pant U 31-AVT, KUG 503, VBG 09-005 and NDU 11-202 were identified as the early flowering while DBGV 5,Vijay and WBB-1 were identified as tall having plant height more than 60 cm. Seed size (wt. of 100 seeds) ranged from 1.36 g in WBB-1 to 6.30 in COBG 759. The number of alleles per SSR marker varied from 3 to 5 per locus. Polymorphic information content values (PIC) ranged from 0.221 to 0.682 per locus. Variability among groups (FIS=0.981) and variability within individuals (FIT=0.986) was low. The most informative loci were CEDG 180, CEDG 139 and CEDG 279 with 5 al¬leles, CEDG 006, CEDG 143 and CEDG 056 with 4 alleles and CEDG 282, CEDG 204, CEDG 118, CEDG 068, CEDG 008, CEDG 043 with 3 al¬leles each. The minimum and maximum molecular genetic distances were found to be 0.00 (PU 08-2 with AKU 10-1) and 1.00 (NDU 11-202 with AKU 10-1, SB 44-4) respectively. Genotypes WBB-1, VBG 09-005 and IPU 10-17 showed high level of genetic diversity. Blackgram improvement through hybridization by utilizing diverse genotypes is suggested for breeding suitable genotypes for North Eastern region. Key words: Fixation index, Genetic diversity, genetic distances, SSR


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