Comparitive HPTLC Determination of Phenolic Acid Profile in Three Polygonum Species.
Publication Date : 06/12/2016
HPTLC analysis was carried out on phenol compounds profile in the whole-plant samples of selected Polygonum species (P. chinense, P. glabrum and P. barbatum). The methanol extract of whole-plant samples obtained from Polygonum species (P. chinense, P. glabrum and P. barbatum) showed 15, 11 and 12 compounds, respectively, and were compared with catechin and quercetin standards. Among the compounds, 6, 6 and 3 compounds in each sample, respectively, were identified as phenols while the others were unknown. Four (two unknown and two phenol) compounds from each of P. chinense and of P. glabrum showed same peak Rf values (0.01, 0.36, 0.47 & 0.96). Similarly, two (one unknown and one phenol) compounds of P. chinense and P. barbatum were also showed same peak Rf values (0.26 & 0.96). On the other hand, two (one unknown and one phenol) compounds of P. glabrum and of P. barbatum showed similar peak Rf values (0.21& 0.96), while all other compounds are differ from each other. The one unknown compound with Rf value 0.98 found in three Polygonum species tested. All the Polygonum species tested shows variations in the nature and number of phenol compounds detected by HPTLC analysis. Key words: Polygonum species, Whole plant samples, Methanol extracts, Phenol compounds, HPTLC analysis.
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