Effect of iron stress on oxidative metabolism in wheat plants (Triticum aestivum (L).
Publication Date : 09/12/2016
Wheat plants (Triticum aestivum L. var. DBW 17) exposed to different concentration of iron(10, 100, 200, 400 µM Fe) in the form of FeEDTA under controlled glass house conditions were quantified for different physiological parameters and antioxidative enzymes as well as antioxidant compounds. At 40 and 60 days of exposure the plants were harvested and growth, active Fe content, lipid peroxidation enzymes and metabolites of the antioxidative metabolism were determined. Plants showed maximum growth at 100 µM Fe supply and this level was treated as control. At 10 µM Fe plants shoewd maximum reduction in growth and choruses in young leaves. Excess of Fe also caused significant inhibition of growth and induced bronzing of older leaves. High concentration of TBARS (indicated lipid peroxidation) and H2O2 content in leaves were detected in Fe deficient and Fe toxic plants (10, 200 and 400 µM Fe) as compared to control. The activities of Fe containing enzymes such as speroxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX) increased with increasing Fe concentration at 40 DAT. A significant enhancement in the activity of GR was also observed with increasing Fe concentration at 40 DAT but the activity of GR as also that of POD and APX reduced at 60 DAT. The ascorbate (ASA) and non- protein thiol (NPT) content in general increased with increasing Fe concentration. The results indicate that under Fe stress condition plants suffer increased oxidative damage, which is regulated by change in the activities of antioxidative enzymes and the contents of the antioxidants ASA and NPT.
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