A RAPID AND SIMPLE METHOD FOR THE ISOLATION OF TOTAL DNA FROM Trichoderma spp.
Publication Date : 17/03/2016
DNA isolation from filamentous fungi is often tedious, time consuming and difficult because they have cell walls that are relatively unsusceptible to lysis. Here described a rapid, inexpensive and reproducible protocol for the isolation of DNA from the filamentous fungus, Trichoderma spp. The present protocol is based on the sodium dodecyl sulfate method without using β- mercaptoethanol and devoid of liquid nitrogen for the maceration. The precipitation of DNA was done using isopropanol and ethanol. The A260/280 absorbance ratio of isolated DNA was ≈1.9 indicating that DNA fraction was pure and could be used for further analysis. The result of DNA electrophoresis in 0.8% agarose gel showed sharp clear bands of DNA, indicated the good quality of DNA. Since this protocol yielded the genetic DNA in sufficient quality and quantity this can be used for the successful isolation of DNA from Trichoderma spp.
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