ANTIBACTERIAL AND PHTYOCHEMICAL ANALYSIS ON Brassica oleracea var. botrytis Linn.
Publication Date : 06/03/2016
ABSTRACT Objective: To investigate the antibacterial activity and phytochemical analysis of Brassica oleraceae. Methods: The antibacterial activity was evaluated using agar well diffusion and microdilution methods against the bacterial (E.coli, Proteus, S.aureus, Klebsiella and Pseudomonas) iolates. The extraction of the vegetable was carried using the solvent namely acetone. Phytochemical, FT-IR and HPLC analysis was carried out in the acetone extract. Results: Acetone extract showed maximum activity against E.coli, Proteus, S.aureus, Klebsiella and Pseudomonas. Phytochemical analysis of Brassica oleraceae extracts showed the presence of secondary metabolites like alkaloids, flavonoids, glycosides, phenolic compounds, saponins, tannins, terpenoids, carbohydrates and anthraquinone. The FT-IR and HPLC revealed different characteristic peak values with various functional compounds in the extracts. Conclusion: From this study, it can be concluded that Brassica oleraceae exhibits antibacterial activity against certain microorganisms. Keywords:Brassica oleraceae, Brassicaceae, Antibacterial, Phytochemical analysis, HPLC and FT-IR analysis
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