Bioassay of entomopathogenic fungi, Beauveria bassiana (Bals.)Vuill and Metarhizium anisopliae (Metchnikoff)Sorokin.,against Sweet potato tortoise beetle, Metriona circumdata H.

Publication Date : 08/03/2016


Author(s) :

Dr.Anis Joseph. R , Dr.Sudharma.K.


Volume/Issue :
Volume 2
,
Issue 3
(03 - 2016)



Abstract :

Bioassays with entomopathogenic fungi, B. bassiana and M. anisopliae on sweet potato tortoise beetle, M. circumdata were conducted to assess the LC50, LC90 and LT50 values and fiducial limits and regression parameters. The results of the study indicated that the mortality of the insects was dependent on the concentration of the spore suspension of the fungus. The cumulative per cent mortality varied with dose and time for both adult beetles and grubs for both the fungi. The dose and time taken for mortality of the adults and grubs of M. circumdata was lower for B. bassiana when compared to M. anisopliae. The LC 50 of B. bassiana estimated were 7.26 × 109and 4.96 × 108 spores ml-1 against the adults and grubs of M. circumdata respectively and a spore concentration of 6.13 × 1010 and 4.64 × 109 spores ml-1 of M. anisopliae was required to cause 50 per cent mortality of the adults and grubs of M. circumdata respectively at the highest test dose evaluated.


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