IN VITRO ANTAGONISM OF Trichoderma spp. AGAINST SIX SOIL BORNE FUNGAL PATHOGENS

Publication Date : 11/03/2016


Author(s) :

V. M. Hima , S. Beena .


Volume/Issue :
Volume 2
,
Issue 3
(03 - 2016)



Abstract :

The concept of biological control is getting more importance nowadays than the chemicals because of its ecofriendly nature. The most popular bio-control agent Trichoderma spp. is known for its antagonistic property against various soil borne fungal pathogens. By taking these into consideration an in vitro study was conducted with twenty native isolates of Trichoderma spp. isolated from soil samples collected from different locations of Kerala and important soil borne fungal pathogens viz., Pythium aphanidermatum, Phytophthora capsici, Fusarium oxysporum, Sclerotium rolfsii, Ganoderma lucidum and Rhizoctonia solani. The antagonistic efficiency of these isolates was tested by taking two released isolates of Trichoderma spp. viz., T. viride and T. harzianum of Kerala Agricultural University, as reference. The results revealed that all the twenty tested native isolates of Trichoderma spp. were efficient in controlling the pathogens, viz., P. aphanidermatum, G. lucidum, S. rolfsii and F. oxysporum than that of reference cultures. The isolates Tr9, Tr 48 and Tr 52 showed cent per cent inhibition over the pathogen, P. aphanidermatum. All the native isolates of Trichoderma spp. recorded 83.33-100 per cent inhibition on the growth of the pathogen, P. capsici. Except 3 isolates viz., Tr 28 (44.85%), Tr 76 (57.78%) and Tr 114 (58.18%), all showed higher antagonistic efficiency to G. lucidum than that of reference cultures. The native isolates recorded 50 per cent inhibition on the growth of S. rolfsii whereas T. viride and T. harzianum showed a per cent inhibition of 28.11 and 35.22 respectively. All except Tr 41, Tr 78, Tr 81 and Tr 118 recorded cent per cent inhibition over F. oxysporum. Among the twenty native isolates of Trichoderma spp. tested, twelve isolates which were able to show higher antagonistic potential than the reference cultures of KAU, can be used for further use.


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