PHYTOCHEMICAL SCREENING OF ROOT EXTRACT OF PHYLLANTHUS FRATERNUS WEBSTER.
Publication Date : 14/03/2016
Phyllanthus fraternus Webster , a pan tropical weed originated from western India, belongs to family Euphorbiaceae . Medicinally it is used by tribals of Gujarat to cure certain diseases like asthma, cough,diarrhea, diabetes, skin diseases and scabies. The preliminary phytochemical study of extract of root using methanol as a solvent was performed.The study revealed the presence of tannins, alkaloids,saponins,flavanoid,terpenoid and steroid. Quantification of isolated compounds of root extract were made using HPTLC and HPLC.Extracts of isolated compound PF-1 and PF-2 were dissolved in both chloroform and methanol. Methanol extract containing isolated compound PF-1 and PF-2 were scanned at 254 nm and 366 nm.The present study revealed that isolated compounds contain medicinally important bioactive compounds result shows maximum antimicrobial activites of root extract against Pseudomonas aeruginosa and Salmonella typhi B with highest inhibition zone So far There is no report on isolated compounds of this plant species. Key word : Methanol root extract, HPTLC,alkaloid , HPLC , zone of inhibition
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