STANDARDISATION OF COPROANTIGEN DOT ELISA FOR DIAGNOSIS OF BOVINE AMPHISTOMOSIS
Publication Date : 04/04/2016
Amphistomosis is a snail borne trematode disease in ruminants causing high morbidity and mortality in tropical and subtropical areas causing great economic losses. The disease is caused mainly by immature flukes and seasonally egg producing trematodes which makes conventional ova detection inefficient. Early diagnosis using coproantigens is a novel method showing good promise. The present study was carried out to standardise a rapid field level diagnostic test for early detection of coproantigens in amphistomosis. Hyper immune polyclonal antisera raised in rabbits against excretory secretory antigens of amphistomes were used in the study for coproantigen detection. Out of the fifty known amphistome ova positive samples, 38 gave positive brown dots indicating 76 per cent infection. Twelve positive samples turned negative in Dot ELISA which may be due to the low fluke burden in these animals thus excreting undetectable coproantigens in faeces. Immunodot did not give any false positive reaction and had 100 percent specificity, 76 per cent sensitivity, 100 per cent positive predictive value (PPV) and 62.5 per cent negative predictive value (NPV) with a accuracy of 83 per cent. Use of monoclonal antibodies and more purified antigens can increase the sensitivity of the present test and can be developed to a commercial rapid dipstick ELISA for early diagnosis of amphistomosis.
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