Molecular Identification of Streptomyces clavuligerus and Structure Elucidation of Its Antimicrobial Efficiency
Publication Date : 04/04/2016
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Abstract :
The present study was carried out for screening antimicrobial substance produced by soil Actinomycetes that demonstrated inhibitory effects against pathogenic microorganisms. Eleven Streptomyces sp. isolates were tested as antimicrobial producers, the most potent producer was isolate encoded C10S19 and was identified by molecular techniques as Streptomyces clavuligerus .The ethyl acetate extract was assayed by paper disk method and purified using thin layer chromatography (TLC). The structure elucidation of antimicrobial agent was performed using ultraviolet (UV), Infra-red (IR) and Mass spectra. Results revealed that the antagonistic activity of clavulanic acid salt and holomycin produced by Streptomyces clavuligerus were active against Gram positive Staphylococcus aureus ATCC 29213, Gram negative Escherichia coli ATCC 25922 and unicellular fungi Candida albicans ATCC 10231.
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