Effects of Temperature and pH on the Morphology of Bio-polymeric Chitosan-alginate Nanoparticles Loaded with Coffee White Stem Borer Pheromone
Publication Date : 06/07/2016
Effects of temperature and pH on the morphology of bio-polymeric chitosan-alginate nanoparticles loaded with Coffee white stem borer (CWSB) pheromone were studied. The nanoparticles were prepared by the ionotropic pre-gelation of alginate with calcium chloride, followed by the complexation between alginate and chitosan. The influence of temperature, pH, and the stoichiometric relationships between the polyelectrolytes were assessed by photon correlation spectroscopy (PCS), using a Zeta PALS particle size analyzer. The data when subjected to analysis of variance (ANOVA) showed that at various pH readings (6, 9, 10, 11 and 12) and with temperatures over a range from 6 to 70 0C, there were no effects on nanoparticle size. Similarly, the zeta potential analysis results for the chitosan-alginate nanoparticles (CS-ALG NPs) loaded with the pheromone with a zeta potential at pH range 6 - 12 were not significantly correlated when subjected to ANOVA. The studies indicated that generally pH has a non-significant effect on zeta potential. However, at pH 8 the effect of temperature was found to be significantly correlated with particle size. This study helps in understanding the determination of the size of nanoparticles and their loading capacity for pheromones.
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