Genetic manipulation of some bacterial strains for improvement of PUFA production
Publication Date : 25/07/2016
Polyunsaturated Fatty acids (PUFA), classified as omega-3 and omega-6 families, are essential fatty acids as these cannot be synthesized de-novo and must be supplied in the diet. A cursory look at literature demonstrates the positive role of ω-3 fatty acids in alleviating variety of life style and life threatening diseases and health conditions. In the present study 3 bacterial strains were subjected to random mutagenesis using UV irradiation and MMS (Methylmethanesulfonate) treatment for improvement of PUFA production. Variety of mutants obtained after exposure to mutagens with varied time or concentration demonstrated that random mutagenesis can be a simple, quick and promising technique for obtaining mutants either with improved or modified PUFA content as judged by the GC analysis. Fourteen mutants with interesting PUFA composition or increased amount of PUFA production were identified. Mutants B. sps. FPZSP13/UV/1, B. sps. FPZSP13/UV/2, H. sps.QY113/UV/2, H. sps.QY113/MMS/1, B.t 407/UV/1 and B.t 407/MMS/2 produced significantly higher amounts of a single or variety of PUFA like Linoleic acid, Alpha-linolenic acid, Eicosatrienoic acid, etc. Further characterization of such mutants revealed that mutantsH. sps.QY113/UV/2 and B.t 407/MMS/2 also have improved biomass production. Further mutagenesis program with these mutants coupled with media and process optimization can be employed to develop few of them into commercially promising mutants to produce either single PUFA or mixture of useful PUFA. Keywords- PUFA, ω-3 fatty acids, ω-6 fatty acids, Random Mutagenesis, UV, MMS, GC.
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