Effect of cultural media and temperature on growth and sporulation of Colletotrichum truncatum of soybean in vitro

Publication Date : 03/01/2017

Author(s) :

Chavan Smita , Dhutraj D.N..

Volume/Issue :
Volume 3
Issue 1
(01 - 2017)

Abstract :

Abstract: Among the fungal diseases infecting soybean crop, anthracnose/pod blight caused by Colletotrichum truncatum is one of the most important and destructive disease. In the present investigation ,the rate of growth of Colletotrichum truncatum has been compared in various culture media and temperature. The diversity in cultural characteristics viz., colony diameter, mycelial growth and sporulation of C. truncatum was studied in vitro using ten culture media. Of the culture media tested significantly highest (90.00 mm) growth was recorded on PDA, followed by Oat Meal Agar (82.20 mm) and Czapek’s dox Agar (80.23 mm).The maximum [excellent (++++)] sporulation was found in Potato Dextrose Agar, Oat Meal Agar, Richard’s Agar; whereas, media viz., Czapek’s dox Agar, Modified Czapek’s dox Agar, Sabouroud’s Agar and V8 Juice Agar exhibited fair (++) sporulation. The test fungus exhibited differential response to various temperature regimes and pH levels tested. The studies indicated that the test fungus could grow in a wide temperature range of 10-40oC; however, it could proliferate better with significantly highest mycelial growth of 82.40 and 77.80 mm at temperatures of 25 and 30oC, respectively which also exhibited good (+++) to excellent (++++) sporulation. Lowest mycelial growth recorded at temperature of 10oC (21.50 mm) and 40oC (16.20 mm).This is important for further study of disease management. Key words: Soybean, temperature regimes ,Colletotrichum.,sporulation

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