Comparative effectiveness of synthetic fungicide and Neem seeds aqueous extract on Colletotrichum gloeosporioides, yam anthracnose disease causative agent

Publication Date : 31/10/2017

DOI : 10.22623/IJAPSA.2017.3117.PITNV


Author(s) :

Kouassi Francis YAO , Patrice Kouamé ASSIRI , Daniel Kouamé KRA , Hortense Atta DIALLO.


Volume/Issue :
Volume 3
,
Issue 10
(10 - 2017)



Abstract :

Anthracnose is economically the most damaging yam disease in the course of its growth against which no fungicide is approved. The objective of this work is to compare the effectiveness of synthetic fungicide against neem seeds aqueous extract on Colletotrichum gloeosporioides, yam anthracnose causative agent. The only stock solution of synthetic fungicide (SF) was compared with three stock solutions of neem aqueous extract (NS1, NS2 and NS3) macerated for 24 and 48 hrs. Three concentrations were separately prepared by incorporating 10; 25 and 50 % of each stock solution in the culture medium for in vitro testing. 45 µL of fungicide and neem extract were applied separately to fungi inoculum used to infect yam detached leaves. Fungi mycelial growth and conidial germ tube elongation were completely inhibited by fungicide. However, they were gradually inhibited according increasing neem aqueous extract concentrations. Neem stock solution NS3 macerated at 48 h showed similar effect with the fungicide in vitro (P ˃ 0.05). Fungi inoculum treated with synthetic fungicide and 25 and 50 % of NS3 did not induce any necrosis symptoms, five days after inoculation. This experiment constitutes a basic study for future yam anthracnose management on yam farms.


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