On the possible effects of Pseudomonas syringae pv. Syringae in Vigna radiata [L.] special reference to photosynthetic pigments and biochemical constituents
Publication Date : 23/02/2017
ABSTRACT Pseudomonas syringae pv. Syringae is one of the mainly intensively consider bacterial plant pathogens. An attempt was made to induce pathogenesis by injecting Pseudomonas syringae in to the stem region of 15-d-old green gram seedlings. Manual infection was found to be successful in inducing symptoms on the leaves. The infected leaves were subjected to photosynthetic pigments and biochemical constituents. It was found that the photosynthetic pigments were highly affected especially chl and carotenoids. Nearly 75% reduction in total chl was noticed. On the contrary, the non-photosynthetic pigments viz., anthocyanin and flavonoids got increased upon infection. Biochemical constituents such as soluble protein, NR activity, phenol content, catalase, peroxidase activity were also increased in infected leaves. From the inoculation site, lysis occurred on the epidermal cells, palisade and spongy parenchyma cells, with strong staining of the cellular contents with abnormal intercellular spaces in the palisade and spongy parenchyma, hypertrophy and hyperplasia of mesophyll cells and partial destruction of chloroplasts. The results are useful to understand the plant-pathogen interaction which causes a serious damage to plant growth and yield. Key words: Anthocyanin, Catalase, Flavonoids, NR activity, Peroxidase, Pseudomonas syringae, Chlorophyll, Vigna.
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