Bacillus manliponensis – A new member of Bacillus cereus group isolated from marine algae Enteromorpha intestinalis (L) Nees (Chlorophyceae)

Publication Date : 12/04/2017

DOI : 10.22623/IJAPSA.2017.3018.YZIX3


Author(s) :

Emeemal Sharon .S , Rani.G.


Volume/Issue :
Volume 3
,
Issue 4
(04 - 2017)



Abstract :

New Bacillus sps, strain BL4-6T was isolated from the green marine algae Enteromorpha intestinalis (L) Nees (Chlorophyceae). BL4-6T cells are oxidase-positive, catalyse-positive, ammonia production-positive. Based on the comparative 16S rRNA gene sequence analysis, the isolate belongs to Bacillus cereus group and it is closely related to Bacillus cereus(97.99%), Bacillus anthracis(97.99%), Bacillus thuringiensis(97.81%), Bacillus toyonensis(97.81%), Bacillus mycoides(97.45%), Bacillus weihenstephanensis(97.45%). The growth of this nitrogen fixing bacteria is proved by the formation of pellicle in nitrogen deficient NFB medium.The utilization of different carbon substrates by the bacterium and the effect of various antibiotics on the bacterium is also studied.This is the first report on the occurrence of Bacillus manliponensis as an endophytic nitrogen fixing bacteria in marine algae Enteromorpha intestinalis (L) Nees (Chlorophyceae). Keywords: Bacillus manliponensis, Nitrogen fixing bacteria, Bacillus cereus group, Liquid Sea weed fertilizer.


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