Microbial source tracking through antibiotic resistance indexing of Escherichia coli to identify source of fecal contamination in drinking water
Publication Date : 23/04/2017
Fecal pollution of water leads to introduce variety of intestinal pathogens that causes water borne diseases. Microbial source tracking (MST) methods are increasingly being used to identify fecal contamination sources in drinking water A total of 400 drinking water samples were collected from the houses (200), schools (100), hostels (50) and commercially available water cans (50), out of which 160 water samples were found nonpotable. A total of 154 E.coli were isolated and identified from these 160 nonpotable drinking water samples. The 123(79%) from human fecal origin and 31(21%) animal fecal origin E. coli were found after the microbial source tracking by multiple antibiotic resistances (MAR) analysis. In hostels and School’s water identified a total of 19 and 18 human fecal originated E.coli. Out of 12 E.coli isolated from 15 Can’s nonpotable water; 10(83%) were human and 2(17%) animal fecal origin. The 105 E.coli were isolated from the household drinking water out of these 76(72%) was human and 29(28%) was animal fecal origin. The study concluded that the water in urban area was mainly polluted due human fecal origin as compare to animal fecal origin.
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