A Comparitive Study Of The Effect Of SA And EMS On In Vitro Production Of Andrographolide In Andrographis paniculata (Burm.F.) Nees

Publication Date : 29/08/2017

DOI : 10.22623/IJAPSA.2017.3086.EHUBN


Author(s) :

Anitha Chandran N , Susila Kuruvilla , P R Unnikrishnapillai.


Volume/Issue :
Volume 3
,
Issue 8
(08 - 2017)



Abstract :

Comparative effects of 0.01% of EMS and 0.01% SA (sodium azide) for 3 hours on secondary metabolite production in Andrographis paniculata were conducted in this study. Calli were derived from leaf explants inoculated on Murashige and Skoog’s (MS) medium supplemented with 2 mg/l NAA, 1 mg/l Kinetin and 50 mg/l Phenylalanine. Treatment of 0.01% SA for 3 hours resulted in 7 fold increase in andrographolide production when compared to control and 0.01% EMS for 3 hours produced 6 fold increase in andrographolide production compared to the control. The study also showed that 0.01% SA for 3 hours treated samples positively influenced on the fresh weight, dry weight of calli, callus induction frequency and andrographolide production compared to the 0.01% EMS for 3 hours treated samples.


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