ARTIFICIAL INSEMINATION OF EMU SEMEN DILUTED WITH IMV AND MODIFIED BELTSVILLE POULTRY SEMEN EXTENDERS (MBPSE)
Publication Date : 05/03/2019
A study was conducted for insemination of emu semen diluted with IMV poultry semen extender (IMV) and Modified Beltsville Poultry Semen Extender (MBPSE). Ten adult male and fifteen female emu birds aged 3 to 4 years were selected and housed individually in emu unit, University Research Farm, TANUVAS, Chennai – 600051. The male birds were selected based on their readiness in accepting human beings without fear. All the birds were housed properly under standard managemental condition. An Isocaloric and Isonitrogenous standard emu breeder ration was fed to birds and portable drinking water were made available ad libitum. The selected male emus were trained for semen collection by non-teaser method. After collection, the raw semen was diluted with IMV and MBPSE semen extenders at 1:3, 1:4 & 1:5 ratio and stored at 4oC and evaluated all the seminal attribute in 1, 3 and 6 hours interval. The semen diluted 1:3 & 1:4 ratio stored for 1 hour showed better seminal characteristics and chosen for insemination. The female birds were restrained by casting on the floor; the artificial insemination was carried out with diluted semen. The female started laying eggs three to four day after insemination. All the eggs were set for incubation. A significant (P<0.01) difference was observed in fertility of female emu birds inseminated with extended semen and the IMV showed better fertility and hatchability than MBPSE and the embryonic mortality did not showed any difference. It is concluded that the emu semen can be stored for a short period of 1 to 3 hour at 1:3 & 1:4 ratio of LSE in 4oC with better seminal attributes for further processing like artificial insemination and cryopreservation.