Effect of 2,3,7,8 tetrachlorodibenzo-p-dioxin in the total cholesterol and oestrogen content of MCF-7 cells
Publication Date : 11/03/2020
Dioxins are persistent organic pollutants that modulate steroidogenesis in cells by affecting their production and metabolism. The effect of 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) treatment on oestrogen and total cell cholesterol was studied in MCF-7 cell lines. MCF-7 cells were grown in RPMI 1640 media containing 10 per cent serum and one percent of antibiotic antimycotic solution. The cells were grown in six well plates and exposed to 1, 3.1 10 and 100 nM concentrations of TCDD for 96 hours. The cells were harvested every 24 hours, the total cholesterol isolated using isopropanol: n-hexane and was quantified by spectrofluorimetry using Amplex Red assay kit. The media collected every 24 hours were used for estimation of total oestrogen content using ELISA. The results of the study revealed that the total cell cholesterol levels were maximum in cells treated with 3.1nM TCDD after 96 hours MCF-7 cells. The cholesterol concentration in cells was lowest in cells treated with 10nM concentration of TCDD. The cells treated with 100nM concentration of TCDD did not survive for more than 24 hours. Hence it could be concluded that higher concentrations of TCDD caused a decrease in total cell cholesterol whereas lower concentrations caused deposition of cholesterol in cells.