Standardization of suitable medium for native endophytic entomopathogenic fungus, Fusarium oxysporum Schltdl.

Publication Date : 12/12/2020

DOI : 10.22623/IJAPSA.2020.6017.YIQJH

Author(s) :

Smitha Revi , Madhu Subramanian.

Volume/Issue :
Volume 6
Issue 12
(12 - 2020)

Abstract :

The effect of mycological media on mycelial growth, sporulation, viability of spores and fungal biomass production of Fusarium oxysporum, a native endophytic entomopathogenic fungus was studied. The nutritional requirement varies with the entomopathogenic fungal species and even the fungal strain. In this study, five different media were used and significant variability was observed on different media. Isolate produced maximum mean mycelial growth on SMA medium followed by SMAY and PDA. PDA medium supported maximum sporulation (1.72 x 105spores ml-1) and viable spore count (5x105 cfu ml-1). Maximum mean dry fungal biomass was observed in SDYB (5.811 g). Sporulation is favoured by nutritional conditions that restricted the fungal biomass production in PDB, which recorded significantly lower fungal biomass (1.345 g). Potato dextrose medium was found to be significantly superior and best suited among all media in the present investigation.

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