A STUDY ON MOLECULAR IDENTIFICATION OF ORCHID MYCORRHIZAL FUNGI OF NERVILIA CROCIFORMIS
Publication Date : 30/01/2022
Present study aimed on identification of orchid mycorrhizal fungi (Nervilia crociformis) using molecular techniques. The terrestrial orchid, Nervilia crociformis, was collected from natural habitats from various locations of Kemmangundi and Sagar, Karnataka State and were investigated for mycorrhizal association. Freshly collected root/tuber of Nervilia crociformis was used for isolation and identification. Pure cultures were obtained by hyphal tip method and were maintained in PDA slants. Pure cultures were used for isolation of genomic DNA and the isolated DNA was run in agarose gel electrophoresis. Polymerized chain reaction (PCR) was performed. The purified PCR product sequences were determined by the chain termination method using an ABI 3130 Genetic Analyzer. The ITS sequence data of the isolate from the tuber of Nervilia crociformis showed close resemblance to Colletotrichum fruticola (Gene Bank Accession No.: KC920479). There was 98% homology to the above fungus along with Glomerella cingulate (perfect stage of the above fungus). Colletotrichum fruticola was found in Nervilia crociformis and they have formed pelotons in cortical cells of the tuber. Thus, the molecular identification study confirms that fungi Colletotrichum fruticola was isolated from tuber of Nervilia crociformis.
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